Yeast-based production, purification and bioactivity assay of rainbow trout LECT2
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چکیده
منابع مشابه
Purification and assay of the budding yeast anaphase-promoting complex.
The anaphase-promoting complex (APC) is a central regulator of the eukaryotic cell cycle and functions as an E3 ubiquitin protein ligase to catalyze the ubiquitination of a number of cell cycle regulatory proteins. The APC contains at least 13 subunits in addition to two activator subunits, Cdc20 and Cdh1, that associate with the APC in a cell cycle-dependent manner. This chapter describes meth...
متن کاملRainbow Trout, Oncorhynchus mykiss
Rainbow trout are classified as Oncorhynchus mykiss, and as such belong to the same genus as Pacific salmon, and to the family Salmonidae, which includes Atlantic salmon (Salmo salar), various trout (Salvelinus sp.), Arctic char (Salvelinus alpinus), Arctic grayling (Thymallus arcticus) and whitefish (Coregonus sp.). Rainbow trout are native to areas around the North Pacific Ocean, from souther...
متن کاملCryopreservation of rainbow trout blastoderm
In the present study, we successfully cryopreserved entire blastoderms of rainbow trout, Oncorhynchus mykiss, embryos being at around midblastula stage of development. The method is a potential alternative to cryopreserving the whole fish embryo, not successful so far. key words. Blastoderm Blastomeres cryopreservation embryonic cells Trout. Cybium 2008, 32(2) suppl.: 139-141. (1) Faculty of Bi...
متن کاملPartial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver
Cyanide is one of the most toxic substances present in a wide variety of food materials that are consumed by animals. Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction. In this study, rhodanese was partially purified and characterized from the liver tissue homogenate of the rainbow trout. The enzyme was active in a broad range of pH, from 5 to 1...
متن کاملGlutathione S-transferases from rainbow trout liver and freshly isolated hepatocytes: purification and characterization.
Glutathione S-transferases (GST) form an important family of biotransformation enzymes catalyzing the conjugation of glutathione to a great variety of xenobiotic compounds. The objective of this study was to compare the different characteristics of GST from freshly isolated rainbow trout hepatocytes with those corresponding to the total liver of the same fish, in order to establish the similari...
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ژورنال
عنوان ژورنال: Zoological Research
سال: 2013
ISSN: 0254-5853
DOI: 10.3724/sp.j.1141.2013.01033